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Title: Biochemical and mechanical analysis of decellularized oesophagus for oesophageal tissue engineering
Authors: Myint Zu Myaing.
Keywords: DRNTU::Engineering::Bioengineering
Issue Date: 2009
Abstract: A number of decellularization methods have been developed to produce acellular xenogenic extracellular matrix (ECM) scaffolds to reconstruct oesophageal defects due to either congenital or acquired diseases. However, the effects of decellularization process on the resulted ECMs have not been fully elucidated yet. The aim of this project is to produce acellular porcine oesophageal scaffolds for oesophageal tissue engineering application. In this study, two different decellularization methods were established; (1) a chemical-based process (using peracetic acid), and (2) a chemical-mechanical based process (combination of peracetic acid and mechanical scraping). The developed decellularization processes were followed by additional hydrogen peroxide wash step. Optimal parameters were based on qualitative (histology and scanning electron microscopy) and quantitative (DNA and collagen assays, mechanical strength tests) studies. Results showed that the chemical-based decellularization process appears to be more effective in removing cellular elements and preserving ECM of porcine oesophagi than the chemical-mechanical based process. Furthermore, mechanical properties were mostly retained in the decellularized ECM scaffolds derived from chemical-based process.
Description: 123 p.
Rights: Nanyang Technological University
Fulltext Permission: restricted
Fulltext Availability: With Fulltext
Appears in Collections:MAE Theses

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