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Title: Effect of antimicrobial agents on biofilm cells and invitro development of resistance in planktonic cells of pseudomonas aeruginosa PA01
Authors: Balasankar Meera Priyadarshini
Keywords: DRNTU::Engineering
Issue Date: 2009
Abstract: The antimicrobial activity of western as well as eastern drugs against infections caused by P. aeruginosa has become an important area of study in recent years. This study revolves around the use of an effective western drug (Wl), a plant derived chemical or Eastern antibiotic (El) and their combinations against PA01 strain of P. aeruginosa. The synergistic effect of these drugs against biofilm formation was determined using quantification assay technique and their visual evidence was confirmed by Scanning Electron Microscopy. The results of this study indicate that biofilm formation is inhibited in the presence of El even at a sub minimum inhibitory concentration (MIC) value when compared to Wl treated at the same sub MIC value. This study also confirms the presence of moderate synergism between El and Wl in inhibiting biofilm formation. A parallel experiment involved in the development of resistance by planktonic cells of P. aeruginosa against the same antimicrobial agents was carried out. The results showed promising development of resistance at a very high concentration (10 MIC) of Wl within 3 subcultures, at a moderate concentration (5 MIC) of El within 15 subcultures, both of which were treated separately. Resistance to the combination of these drugs (Wl+El) was developed at a concentration of 5 MIC within 13 subcultures.
Description: 97 p.
Rights: Nanyang Technological University
Fulltext Permission: restricted
Fulltext Availability: With Fulltext
Appears in Collections:MAE Theses

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