Development of scaffolds for oesophageal tissue engineering
Leong, Meng Fatt
Date of Issue2009
School of Mechanical and Aerospace Engineering
Tissue engineering has emerged as a potential solution to replace diseased or defective organs. This project describes the development and characterization of a tissue engineered scaffold that is suitable for the replacement of the oesophagus. Electrospinning is chosen as the fabrication method for the scaffold. Despite the versatility of the electrospinning process in fabricating fibrous scaffold, it suffers from the severe limitation of producing scaffold with microscale pores (<5|J.m) that inhibit cell infiltration. In this project, a technique that uses ice crystals as templates to fabricate cryogenic electrospun scaffolds (CES) with large three-dimensional and interconnected pores using poly(D,L-lactide) (PLA) was developed. The pore sizes were controlled by manipulating the environment's humidity, mandrel temperature and solution flow rate.
Nanyang Technological University