X-ray crystallographic characterization of heavy metal-nucleosome association and nucleosome core particle composed of novel DNA sequences
Mohideen Abdul Kareem
Date of Issue2010
School of Biological Sciences
Metal ions with strong carcinogenic potential, including Cd2+, Ni2+, Co2+ and Cr6+, are known to play a significant role in disrupting a broad range of cellular processes. However, the precise mechanisms by which heavy metals exert their carcinogenic effects are not clear and require further studies. Recent developments suggest that epigenetic mechanisms may play an important role in activating or silencing the expression of genes, which are critical for driving the metal-induced pathogenesis. In our study, using X-ray crystallography, we characterized the binding of Ni2+ and Co2+ to the nucleosome core particle (NCP) - the fundamental entity of chromatin - in order to comprehend their site-selectivity and influence on the structure of nucleosomal DNA.
Nanyang Technological University