Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/48426
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dc.contributor.authorTan, Raymond Yong Tai
dc.date.accessioned2012-04-23T06:44:10Z
dc.date.available2012-04-23T06:44:10Z
dc.date.copyright2012en_US
dc.date.issued2012
dc.identifier.urihttp://hdl.handle.net/10356/48426
dc.description.abstractThe egg capsules of Busycotypus canaliculatus, that protect the developing embryos from adverse environment conditions and predators, have very unique material properties. From previous studies, it was shown that these egg capsules possess high reversible extensibility and stiffness which has rapid recovery upon extension due to reversible phase transition between α-helix to β-sheets. The egg capsule precursors actually undergo self-assembly and crosslinking prior to their achieving mature form, thus, it is important to study these processes that result in possessing these mechanical properties. The egg capsule is assembled from four main Capsule Proteins (CP1a, CP1b, CP2 & CP3) and their amino acid sequences have been deduced by previous researchers. However, the characterizations were incomplete due to inadequate protein yields after extractions and purifications. To tackle this problem, a microbial system using E. Coli (Escherichia Coli) was successfully established to produce these proteins in high yields, eliminating the need for their direct extraction from B. canaliculatus. For the next step of purification, cation exchange, reverse phase and size exclusion chromatographic techniques were used, starting with CP1a. However, many problems surfaced during the purifications and only some were resolved. CP1a was not sufficiently pure to proceed to the next stage, characterization. Nevertheless, the experimental protocols were sufficiently optimized, approaching >90% purity for CP1a. This work paves the way in the future for the rapid purifications of the remaining egg capsule protein, and offers the potential for the creation of robust biomimetic elastomeric materials.en_US
dc.format.extent48 p.en_US
dc.language.isoenen_US
dc.rightsNanyang Technological University
dc.subjectDRNTU::Engineering::Materials::Biomaterialsen_US
dc.titleRecombinant expression and purification of coiled-coil bioelastomeric proteins from marine snail egg capsulesen_US
dc.typeFinal Year Project (FYP)en_US
dc.contributor.schoolSchool of Materials Science and Engineeringen_US
dc.description.degreeBachelor of Engineering (Materials Engineering)en_US
dc.contributor.researchBioMedical Engineering Research Centreen_US
dc.contributor.researchBiological & Biomimetic Material Laboratory @ NTU
dc.contributor.supervisor2Ali Gilles Tchenguise Miserezen_US
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Appears in Collections:MSE Student Reports (FYP/IA/PA/PI)
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