Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/49281
Title: Role of cargo receptors in Synphilin-1 mediated selective autophagy of protein aggregates.
Authors: Lee, Karen Feng Yi.
Keywords: DRNTU::Science
Issue Date: 2012
Abstract: The ANK-1 domain of synphilin-1 has been recently identified to able to impart autophagic clearance signals to certain disease-linked protein inclusions that were previously resistant to clearance by autophagy. To examine whether ANK1 cooperates with known cargo adaptors, p62 and NBR1, in mediating aggrephagy of ANK1-p38 (A38) inclusions, independent and combinatorial ablation of these cargo adaptors was performed in a wild type and p62 knockout (KO) mouse embryonic fibroblast (MEF) system. By monitoring the turnover and levels of various autophagic markers, we found that variable cargo adaptor levels did not seem to have an impact on the general autophagic function in the cells. Aggrephagic clearance examined by the expression of A38 in p62 KO MEFs to induce inclusion formation demonstrated a significant clearance of protein inclusions that differ only slightly from the clearance presented in wild type cells. Thus, our results revealed that the downregulation of the cargo adaptor protein p62 did not exert a significant impact on synphilin-1 mediated clearance of protein aggregates.
URI: http://hdl.handle.net/10356/49281
Rights: Nanyang Technological University
Fulltext Permission: restricted
Fulltext Availability: With Fulltext
Appears in Collections:SBS Student Reports (FYP/IA/PA/PI)

Files in This Item:
File Description SizeFormat 
Karen's Thesis Final.pdf
  Restricted Access
1.16 MBAdobe PDFView/Open

Page view(s)

176
checked on Sep 28, 2020

Download(s)

5
checked on Sep 28, 2020

Google ScholarTM

Check

Items in DR-NTU are protected by copyright, with all rights reserved, unless otherwise indicated.