Please use this identifier to cite or link to this item:
https://hdl.handle.net/10356/49502
Title: | Studies on the host-virus interactions of coronaviruses | Authors: | Zhong, Yanxin | Keywords: | DRNTU::Science::Biological sciences::Microbiology::Virology | Issue Date: | 2012 | Source: | Zhong, Y. (2012). Studies on the host-virus interactions of coronaviruses. Doctoral thesis, Nanyang Technological University, Singapore. | Abstract: | Virus-induced apoptosis and host antiviral innate immunity are key issues in understanding virus-host interactions and viral pathogenesis. Coronavirus infection of mammalian cells induces apoptotic and host innate immune responses. Global gene expression profiles are determined in coronavirus-infected Vero cells by Affymetrix array, using avian coronavirus infectious bronchitis virus (IBV) as a model system, to reveal up-regulation of both pro-apoptotic B cell lymphoma-2 (BCL-2)-antagonist/killer 1 (Bak) and pro-survival myeloid cell leukemia-1 (Mcl-1). Apoptosis occurred earlier in IBV-infected cells silenced with short interfering RNA targeting Mcl-1 (siMcl-1), and was delayed in siBak cells. Up-regulation of RNA helicases from the RIG-I-like receptor (RLR) family, Melanoma differentiation-associated gene 5 (MDA5) and Retinoic-inducible gene I (RIG-I), was also observed in IBV-infected cells, leading to downstream Mitochondrial antiviral signalling (MAVS) adaptor protein activation for interferon induction in response to viral invasion. RIG-I, MDA5 and MAVS also play a part in modulating IBV-induced apoptosis. Bcl-2 family proteins, too, regulates MAVS cleavage during apoptosis, thus further establishing a link between MAVS and intrinsic apoptotic pathway activation in the mitochondria during host innate immunity induction. | URI: | https://hdl.handle.net/10356/49502 | DOI: | 10.32657/10356/49502 | Schools: | School of Biological Sciences | Fulltext Permission: | open | Fulltext Availability: | With Fulltext |
Appears in Collections: | SBS Theses |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
thesis-final.pdf | 6.19 MB | Adobe PDF | View/Open |
Page view(s) 20
695
Updated on Mar 19, 2024
Download(s) 5
651
Updated on Mar 19, 2024
Google ScholarTM
Check
Altmetric
Items in DR-NTU are protected by copyright, with all rights reserved, unless otherwise indicated.