Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/50313
Title: Influence of different chemicals on the accuracy of the protein quantification assays
Authors: Tan, Hui Ying.
Keywords: DRNTU::Engineering::Materials::Biomaterials
Issue Date: 2012
Abstract: Protein quantification assay is a fast and convenient method which is used to quantify extracted protein. Processes prior to extraction of protein involve chemicals which may affect the accuracy of the results. However these influences on the accuracy of the amount of protein quantified are not very well researched. Therefore, in this project, the chemicals involved in the prior processes such as Sodium Sulfide (Na2S), 2-Mercaptoethanol (2-ME), Urea and Tris(2-carboxyethyl)phosphine (TCEP) are experimented with the 2 different Protein Quantification Assays, Pierce 660nm (P660nm) and Bicinchoninic Acid (BCA), to find out the maximum amount or concentration of the chemicals that one can use in the processes. Bovine Serum Albumin is used as the standard for this project. From the results, the chemicals did show influence on the protein quantification and the maximum concentrations of the individual chemicals used for the respective assays are concluded. Furthermore, the possible effect of the influence is also discussed. In addition, both assays results were compared with the manufacturer’s protocol and P660nm assay showed a higher consistency than the BCA, hence P660nm might serve to be a more suitable assay than the latter.
URI: http://hdl.handle.net/10356/50313
Rights: Nanyang Technological University
Fulltext Permission: restricted
Fulltext Availability: With Fulltext
Appears in Collections:MSE Student Reports (FYP/IA/PA/PI)

Files in This Item:
File Description SizeFormat 
Tan Hui Ying Final Report.pdf
  Restricted Access
1.57 MBAdobe PDFView/Open

Page view(s) 50

241
checked on Oct 23, 2020

Download(s) 50

16
checked on Oct 23, 2020

Google ScholarTM

Check

Items in DR-NTU are protected by copyright, with all rights reserved, unless otherwise indicated.