Please use this identifier to cite or link to this item:
https://hdl.handle.net/10356/50471
Title: | Biophysical and structural studies of self-assembled liposome/E-peptide/DNA nanoparticles : correlation with gene delivery | Authors: | Yan, Jiang | Keywords: | DRNTU::Science::Biological sciences::Genetics | Issue Date: | 2012 | Source: | Yan, J. (2012). Biophysical and structural studies of self-assembled liposome/e-peptide/dna nanoparticles : correlation with gene delivery. Doctoral thesis, Nanyang Technological University, Singapore. | Abstract: | Recently, novel DNA compaction agents, ε-oligo(L-lysines) and their branched α-amino-acid substituted derivatives, have been successfully tested as vectors for in vitro gene delivery. The present work exploits a number of newly developed ε-oligolysine-based peptides: ε-(LYRH)K10, ε-(LYH)K10, ε-(LYK)K10, ε-(LKY)K10 and ε-(YKL)K10. ε-oligolysine backbone was synthesized with degree of polymerization 10 and α-amino acid triplets (amino acids in the brackets) were attached to each of the α-amino group of ε-oligolysine (Fig. 1). Since it was found that combined effect of lipids and polycations can greatly enhance transfection efficiency, our novel ε-peptides were used in the combination of cationic liposome DOTAP (1,2-dioleoyl-3-trimethylammonium-propane), resulting in a ternary complex liposome/ε-peptides/DNA (LPD). | URI: | https://hdl.handle.net/10356/50471 | DOI: | 10.32657/10356/50471 | Schools: | School of Biological Sciences | Fulltext Permission: | open | Fulltext Availability: | With Fulltext |
Appears in Collections: | SBS Theses |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
TbsG0702071G.pdf | thesis | 2.47 MB | Adobe PDF | View/Open |
Page view(s) 20
744
Updated on Mar 18, 2024
Download(s) 20
330
Updated on Mar 18, 2024
Google ScholarTM
Check
Altmetric
Items in DR-NTU are protected by copyright, with all rights reserved, unless otherwise indicated.