Development of an immobilization-capture method for optical detection of bacteria

Abstract

There is a need to develop an online bacterial detection system which enables sample preparation, concentration and bacterial detection to be done in realtime. A flow immunosensor approach based on detection of the fluorescence emission has high potential to be developed into an online bacterial detector. In this study, we developed a system of immobilizing fluorescently-labelled antibody onto a glass surface where the cells are captured.

This study aimed to develop an antibody immobilization method to

immobilize fluorescently-labelled antibody specific to the target bacteria (E.coli)

onto the glass surface to capture and label the cells. X-ray photoelectron

spectroscopy (XPS), atomic force microscopy (AFM) and epifluorescence

microscopy studies revealed the feasibility of using aminopropyltriethoxysilane-glutaraldehyde

(APTES-GA) to immobilize antibody onto glass substrates.