Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/50506
Title: Development of an immobilization-capture method for optical detection of bacteria
Authors: Er, Chin Lu
Keywords: DRNTU::Engineering::Environmental engineering
Issue Date: 2012
Source: Er, C. L. (2012). Development of an immobilization-capture method for optical detection of bacteria. Master’s thesis, Nanyang Technological University, Singapore.
Abstract: There is a need to develop an online bacterial detection system which enables sample preparation, concentration and bacterial detection to be done in realtime. A flow immunosensor approach based on detection of the fluorescence emission has high potential to be developed into an online bacterial detector. In this study, we developed a system of immobilizing fluorescently-labelled antibody onto a glass surface where the cells are captured. This study aimed to develop an antibody immobilization method to immobilize fluorescently-labelled antibody specific to the target bacteria (E.coli) onto the glass surface to capture and label the cells. X-ray photoelectron spectroscopy (XPS), atomic force microscopy (AFM) and epifluorescence microscopy studies revealed the feasibility of using aminopropyltriethoxysilane-glutaraldehyde (APTES-GA) to immobilize antibody onto glass substrates.
URI: http://hdl.handle.net/10356/50506
Fulltext Permission: restricted
Fulltext Availability: With Fulltext
Appears in Collections:CEE Theses

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