Please use this identifier to cite or link to this item:
Title: Enantioseparation of dansyl amino acids in capillary electrophoresis using a novel chiral selector : mono-6-deoxy-6-(ethanolammonium)-β-cyclodextrin.
Authors: Selvi Ramaswami.
Keywords: DRNTU::Engineering::Chemical engineering
Issue Date: 2012
Abstract: A positively charged novel single isomer β-CD was synthesized through nucleophilic substitution of Mono-6-tosyl-β-CDs with Ethanolammonium to give Mono-6-deoxy-6- (Ethanolammonium)-β-cyclodextrin tosylate, followed by anion exchange to produce the chiral selector Mono-6-deoxy-6-(Ethanolammonium)-β-cyclodextrin chloride (EtAM-β- CDCl). The cationic CD was analytically characterized and their chiral resolution ability was studied with Capillary Electrophoresis using dansyl (Dns)-Amino acids as model analytes in acidic aqueous BGE. The concentration of the chiral selector and the pH of the BGE were varied extensively from 6 to 15mM and pH (6.5 to 8.5) respectively to understand their influence on the resolution and selectivity of the cationic CD. From the experiment results, it was seen that the chiral resolution increased with increase in CD concentration and reached optimum at 10mM, but decreased with increase in pH, with the prime at pH 6.5. The electrostatic interaction along with the inclusion complex formation seemed to account for the effective separation of the analytes. Further the fivedansyl amino acid mixture was enantioseparated using EtAM-β-CDCl as chiral selector.
Fulltext Permission: restricted
Fulltext Availability: With Fulltext
Appears in Collections:SCBE Theses

Files in This Item:
File Description SizeFormat 
  Restricted Access
813.25 kBAdobe PDFView/Open

Page view(s) 10

checked on Oct 24, 2020

Download(s) 10

checked on Oct 24, 2020

Google ScholarTM


Items in DR-NTU are protected by copyright, with all rights reserved, unless otherwise indicated.