Please use this identifier to cite or link to this item:
https://hdl.handle.net/10356/5176
Title: | Isolation and characterization of thermoactive extracellular protease-producing geobacillus caldoproteolyticus sp. nov. from sewage sludge | Authors: | Tay, Joo Hwa Wang, Jing Yuan Tay, Stephen Tiong Lee Stabnikova Olena Chen, Xiaoge |
Keywords: | DRNTU::Engineering::Environmental engineering::Water treatment | Issue Date: | 2004 | Abstract: | A proteolytic thermophilic bacterial strain, designated as strain SF03, was isolated from sewage sludge in Singapore. Strain SF03 is strictly aerobic, rod-shaped, Gram- positive, catalase positive, oxidase positive, and forms endospores. Strain SF03 grew at temperatures ranging from 35 to 65 OC, pH ranging from 6.0 to 9.0 and salinities ranging from 0 to 2.5%. Phylogenetic analyses revealed that strain SF03 was most similar to Saccharococcus themophilus, Geobacillus caldoxylosilyticus and Geobacillus thermoglucosidasius, with the 16s rRNA gene sequence identity of 97.6%, 97.5% and 97.2%, respectively. Based on taxonomic and 16s rRNA analysis data, strain SF03 was named Geobacillus caldoproteolyticus sp. nov. Production of extracellular protease from strain SF03 was observed in a basal peptone medium and the peptone medium supplemented with different carbon and nitrogen sources. Different levels of protease production were found with different carbon and nitrogen sources. | URI: | http://hdl.handle.net/10356/5176 | Schools: | School of Civil and Environmental Engineering | Rights: | Nanyang Technological University | Fulltext Permission: | restricted | Fulltext Availability: | With Fulltext |
Appears in Collections: | CEE Research Reports (Staff & Graduate Students) |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
CEE-RESEARCH-REPORT_29.pdf Restricted Access | 1.37 MB | Adobe PDF | View/Open |
Page view(s) 20
663
Updated on Mar 28, 2024
Download(s)
3
Updated on Mar 28, 2024
Google ScholarTM
Check
Items in DR-NTU are protected by copyright, with all rights reserved, unless otherwise indicated.