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dc.description.abstractChronic myelogenous leukemia (CML) is a cancer of hematopoietic cells. It is characterized by the production of BCR-ABL fusion protein. The detection of CML is of utmost importance as the survival period of a malignant cancer is very short. This particular type of cancer is detected by blood counts and bone marrow biopsies. This study concentrates on devising a new method of detecting CML cells (K562 cell line). This new method involves designing a microfluidic polydimethylsiloxane (PDMS) platform with nanofibers in the channel to capture the leukemic stem cells. Therefore, this study will include the fabrication of the PDMS platform with CAD designs to illustrate the platform assembly, preparation of nanofibers for the channel and experiments to analyze cell adhesion to various surfaces and coatings and effect of flow rate or shear stress on the adhesion of the suspension cells. The CAD designs are demonstrative to explain the structure, dimensions and the overall assembly of parts to create the nanofiber-based platform. It is found that among the nanofibers, collagen, fibronectin and coated nanofibers, the fibronectin-coated nanofibers and fibronectin coated surfaces are the best substrates for cell adhesion. It is also seen that collagen-coated nanofibers and fibronectin-coated nanofibers each adhere the most cells at two different flow rates.en_US
dc.format.extent68 p.en_US
dc.rightsNanyang Technological University
dc.titleDesign of nanofiber-based platform for leukemia stem cell capture.en_US
dc.typeFinal Year Project (FYP)en_US
dc.contributor.schoolSchool of Chemical and Biomedical Engineeringen_US
dc.description.degreeBachelor of Engineering (Chemical and Biomolecular Engineering)en_US
dc.contributor.supervisor2Mayasari Limen_US
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Appears in Collections:SCBE Student Reports (FYP/IA/PA/PI)
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