New methodologies for the synthesis of proteins and construction of biomolecular assemblies.

Abstract

Protein chemical synthesis has increasely been used for protein structure-function study. Our laboratory has been engaged in the development of new methodologies for protein synthesis and for the preparation of complex biomolecular structural studies. As a general goal of my thesis project, I mainly aimed to make a contribution to the field of protein chemistry. Specifically, in this project we expanded the application scope of native chemical ligation by developing a new method for peptide thioester synthesis by Fmoc SPPS. Furthermore, we also developed two new methods for sequential peptide ligation to synthesize larger protein. At last, we made use of the hybridization property of PNA for preparing dimeric macromolecules and for specific formation of unsymmetric disulfide bonds.