dc.contributor.authorYang, Yanli
dc.date.accessioned2013-11-08T07:57:29Z
dc.date.accessioned2017-07-23T08:37:40Z
dc.date.available2013-11-08T07:57:29Z
dc.date.available2017-07-23T08:37:40Z
dc.date.copyright2013en_US
dc.date.issued2013
dc.identifier.citationYang, Y. (2013). Ternary sensing surface with DNA-based spacer group: characterization, comparison and optimization. Doctoral thesis, Nanyang Technological University, Singapore.
dc.identifier.urihttp://hdl.handle.net/10356/54958
dc.description.abstractIn the past decade, outbreaks of new diseases have brought much fear in the people worldwide. Many lives were lost due mainly to the late detection of these diseases as well as the lack of knowledge to prevent or cure them. To allow fast response to curb the spread of such infections, real-time sensors with high sensitivity and selectivity are required. With the advancement in technology and the need for miniaturization, biosensors based on electrochemistry has proven to be a powerful detection method due to the ease-of-use, low instrumentation cost, possible non-labeling and fast target detection. In an electrochemical detection, the sensitivity of the biosensors is mostly affected by the accessibility of the specific target towards the recognition site of the receptors immobilized on the electrode surface. To reduce the steric hindrances of the target molecules to the receptors, in this case the single-stranded DNA (ssDNA) probes, short organic spacer groups are normally used to modulate the ssDNA probe density on the electrode. In this report, a ternary sensing surface optimized using DNA-based spacer group for the detection of Methicillin-resistant Staphylococcus aureus (MRSA) has been created. As compared to commonly-used organic spacer group, such as 2-mercaptoethanol (ME), 3-mercaptop-1-propanol (MP) and 6-mercapto-1-hexanol (MCH), thymine-based spacer groups (T9) displayed a 10-fold improvement of signal-to-noise in discriminating between complementary DNA (cDNA) and non-complementary DNA (NcDNA) hybridization. Analysis from Surface Plasmon Resonance (SPR), Quartz Crystal Microbalance (QCM-D) and electrochemistry showed a sensing surface of excellent selectivity, optimized at a ratio of 1:1 (probe:T9). On this surface, the ssDNA probes are aligned by the T9 spacer groups and thereby capable of maximizing cDNA hybridization and differentiating with non-specific NcDNA binding. Single-mismatch (SMM) detections have shown to be possible at this optimized ratio, with the ability to differentiate between the SMM at different positions. By creating similar sensing surfaces on gold-deposited microelectrodes, an improvement of the S/N by a factor of 8 was observed compared to the detection using planar gold electrodes, showing capabilities of creating a highly selective and sensitive biosensor with microelectrodes. Attempts were also made to create binary sensing surfaces and comparisons were made with using shorter thymine spacer groups (T6) on both planar and microelectrodes. While all DNA-based spacer lengths were capable of modulating probe density and reducing steric hindrances, sensing surfaces created using the longer T9 spacer groups performed better in terms of S/N at their respective optimized ratio. Improvement in detection was observed for neutral PNA strands as compared to negatively-charged DNA strands at the same optimized ratio using microelectrodes. Despite the general idea that a compact and uncharged layer is more desirable to form a highly selective biosensor based on electrochemistry, I have shown that as compared to the use of organic spacer groups, a more highly selective ternary sensing layer can be formed by using thymine-based spacer groups. Thymine spacers allow the assembly of a less compact sensing surface (with easily accessible domains in between the upright ssDNA probes) for efficient transfer of electrons across the surface, which is important for signal enhancement in an electrochemical biosensor. Furthermore, similar to one of the functions of the organic spacer groups, thymine spacers are also capable of removing the non-specific interactions between the DNA bases and gold due to their highly negative charged nature. In addition, thymine spacers having similar hydrophilicity to the ssDNA probe is likely to form a more homogenous sensing surface than that using the hydrophobic organic spacer groups. Lastly and most importantly, this work initiates and brings insights into the use of an alternative spacer group for the assembly of a sensing layer.en_US
dc.format.extent192 p.en_US
dc.language.isoenen_US
dc.subjectDRNTU::Science::Biological sciences::Biochemistryen_US
dc.titleTernary sensing surface with DNA-based spacer group : characterization, comparison and optimizationen_US
dc.typeThesis
dc.contributor.supervisor2Bo Gunnar Liedbergen_US
dc.contributor.schoolSchool of Materials Science and Engineeringen_US
dc.description.degreeDOCTOR OF PHILOSOPHY (MSE)en_US


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