Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/55060
Full metadata record
DC FieldValueLanguage
dc.contributor.authorItisam Sarangien
dc.date.accessioned2013-12-11T07:12:17Zen
dc.date.available2013-12-11T07:12:17Zen
dc.date.copyright2013en
dc.date.issued2013en
dc.identifier.citationItisam Sarangi. (2013). The role of previously uncharacterized RNA binding proteins in hematopoiesis. Doctoral thesis, Nanyang Technological University, Singapore.en
dc.identifier.urihttps://hdl.handle.net/10356/55060en
dc.description.abstractThe crucial checkpoints that determine T-cell fate along with various differentiation pathways have been genetically defined. Still there are many uncharacterized genes whose roles in thymus and T cell development have yet to be established. One such example is Thymocyte specific cAMP-regulated phosphoprotein (thymocyte Arpp21 or Tarpp). Although TARPP protein is highly expressed in immature T cells and is down-regulated immediately after T-cell receptor (TCR) gene rearrangements, Tarpp KO mice did not have any apparent immunological phenotype. We hypothesized that two other proteins sharing high homology and RNA-binding domains with TARPP, R3h-domain containing protein 1 and 2 (R3HDM1 and R3HDM2) are able to compensate its function in vivo. Through generation of R3hdm1 and R3hdm2 KO mice, we intended to characterize role of these two RNA-binding proteins in murine hematopoiesis. R3hdm1, R3hdm2 and newly derived Tarpp KO (-/-) mice were viable but surprisingly did not have any immunological phenotype. R3hdm1 KO mice showed a reproductive phenotype with reduced litter size and disrupted internal testis architecture. This report provides more information about structure and genetic organization of these genes. Phenotypic analysis of double or triple KO mice will shed light on the function of these R3H domain containing proteins in T-cell physiology.en
dc.format.extent136 p.en
dc.language.isoenen
dc.subjectDRNTU::Science::Biological sciences::Microbiology::Immunologyen
dc.subjectDRNTU::Science::Biological sciences::Molecular biologyen
dc.titleThe role of previously uncharacterized RNA binding proteins in hematopoiesisen
dc.typeThesisen
dc.contributor.supervisorKlaus Erik Karjalainenen
dc.contributor.schoolSchool of Biological Sciencesen
dc.description.degreeDOCTOR OF PHILOSOPHY (SBS)en
dc.identifier.doi10.32657/10356/55060en
item.fulltextWith Fulltext-
item.grantfulltextopen-
Appears in Collections:SBS Theses
Files in This Item:
File Description SizeFormat 
Itisam_Sarangi Full set PhD Thesis.pdfPhD thesis Itisam Sarangi5.35 MBAdobe PDFThumbnail
View/Open

Google ScholarTM

Check

Altmetric

Items in DR-NTU are protected by copyright, with all rights reserved, unless otherwise indicated.