Quantitative analysis of neural stem cell growth and differentiation.
Date of Issue2014
School of Biological Sciences
Institute of Medical Biology
Neural stem cells (NSCs) are self-renewing, multipotent cells in the embryonic or adult brain that can give rise to neurons and glia. NSCs and neural progenitors (NPs) can be propagated in vitro through a natural 3-D culture system known as neurospheres. Here, I report the use of non-invasive image acquisition and processing techniques to gain insight into the 3-D architecture of the neurosphere, characterise neurosphere forming cells (NFCs), develop an imaging-based method to enrich for NSCs, and a high throughput screening (HTS) strategy to study the effects of drugs on NSCs/NPs.