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Title: Structural diversity of G-rich DNA : from telomeric G-quadruplexes to G-junction motifs
Authors: Lim, Kah Wai
Keywords: DRNTU::Science::Biological sciences::Biochemistry
DRNTU::Science::Biological sciences::Biophysics
DRNTU::Science::Chemistry::Analytical chemistry::Nucleic acids
Issue Date: 2013
Source: Lim, K. W. (2013). Structural diversity of G-rich DNA : from telomeric G-quadruplexes to G-junction motifs. Doctoral thesis, Nanyang Technological University, Singapore.
Abstract: Four-stranded G-quadruplex DNA have been implicated in cellular processes and shown to exhibit important functional properties. In particular, putative quadruplex motifs were identified in important genomic loci including telomeres and oncogenic promoters, rendering them promising therapeutic targets. G-quadruplex DNA could adopt diverse topologies, but current knowledge regarding their folding principles remains lacking. This study addresses the conformational heterogeneity of G-quadruplexes from human telomeric and promoter regions through the structural and biophysical characterization on a number of such sequences. In addition, the connectivity of duplex and quadruplex DNA is validated across the diverse geometries of the quadruplex, accompanied by a search for the existence of sequence motifs comprising both duplex and quadruplex elements in the human genome. These findings provide structural insights which could be useful towards the rational design of chemical ligands for therapeutic targeting of genomic G-rich sequences, and pave way for implementation of quadruplex motifs in DNA nanotechnology.
DOI: 10.32657/10356/55392
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:SBS Theses

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