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Title: Biosensors: alcohol and glucose detection
Authors: Liu, Shuhui
Keywords: DRNTU::Engineering::Mechanical engineering::Surgical assistive technology
Issue Date: 2011
Abstract: A unique approach of developing a simple enzymatic technique for detection of analytes, alcohol and glucose, in body fluids was described. The proposed assay system was mainly composed of buffer, 3,3',5,5'-tetramethylbenzidine (TMB), and enzymes. The analytes were bio-catalyzed by the respective enzymes, alcohol oxidase (AOx) and glucose oxidase (GOx) to produce hydrogen peroxide (H2O2). This was followed by the horseradise peroxide (HRP)-catalyzed reaction to oxidize the indicator solution, TMB, producing coloured compounds. The main measurement was the colour change due to TMB-oxidation process, affected directly by the amount of bio-targets present, and indirectly by the concentration of reagents. The application was performed on nitrocellulose strips and cellulose pads, using spot tests. Investigations also focused on enzyme stabilization and shelf-life.
Description: 104 p.
Rights: Nanyang Technological University
Fulltext Permission: restricted
Fulltext Availability: With Fulltext
Appears in Collections:MAE Theses

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