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dc.contributor.authorJose, Asial Ignacioen
dc.identifier.citationJose, A. I. (2014). Novel methods and screens for portein stability engineering. Doctoral thesis, Nanyang Technological University, Singapore.en
dc.description.abstractProtein stability is often a limiting factor in the development of commercial proteins and biopharmaceuticals, as well as for biochemical and structural studies. In this thesis, I present different methods developed with the aim of improving protein stability, either by mutagenesis or by interaction with a protein partner.For protein stabilization through mutagenesis, a new high-throughput colony-based stability screen is described, which is a direct and biophysical read-out of intrinsic protein stability. As the method is generic and activity independent, it can easily be applied to a wide range of proteins. For protein stabilization through interaction with a protein partner, the use of phage display was explored. As an alternative and complement to phage display, I present the development of a new biophysical screen that monitors protein stabilization upon binding in cells and crude extracts, and its use in de novo binder generation using an autonomous VH domain as scaffold.en
dc.format.extent169 p.en
dc.subjectDRNTU::Science::Biological sciences::Biophysicsen
dc.subjectDRNTU::Science::Biological sciences::Biochemistryen
dc.subjectDRNTU::Science::Biological sciences::Molecular biologyen
dc.titleNovel methods and screens for portein stability engineeringen
dc.contributor.supervisorNordlund Paer Lennarten
dc.contributor.supervisorTobias Cornviken
dc.contributor.schoolSchool of Biological Sciencesen
dc.description.degreeDOCTOR OF PHILOSOPHY (SBS)en
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