Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/59087
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dc.contributor.authorLai, Marcel Chang Long
dc.date.accessioned2014-04-22T07:42:07Z
dc.date.available2014-04-22T07:42:07Z
dc.date.copyright2014en_US
dc.date.issued2014
dc.identifier.urihttp://hdl.handle.net/10356/59087
dc.description.abstractLymphocyte-activation gene 3 (LAG3) is a protein that limits the activity of T-cells, which down regulates the immune system. The protein functions can be determined from its structure. However, there is currently no available structure of LAG3 in any database. In order to fully understand its functions, there is a need to model the structure of LAG3. With the rapid growth of computational development, there are various tools available for modeling protein structure. In this project, three current popular tools, I-TASSER, MODELLER and SWISS-MODEL will be employed to predict and evaluate the LAG3 structure. LAG3 structural modeling by I-TASSER and SWISS-MODEL was done using the online interface and modeling in MODELLER was done using a standalone package. After the LAG3 models were initially constructed, PyMOL was used to align those models and the RMSD values were estimated. RMSD helps to compare how much the model deviate from one another. Finally, energy minimization was performed using Swiss-Pdb Viewer to optimize the structures, and comparisons were done among the optimal structures. It is evident from the findings that the I-TASSER model shows the most favorable prediction of the LAG3 structure among the three tools. With the model of LAG3 structure formed, further research can be done on molecular dynamics simulations of the LAG3 protein. Furthermore, different types of antibodies can be researched on to find out which antibodies are able to respond to the LAG3 protein.en_US
dc.format.extent63 p.en_US
dc.language.isoenen_US
dc.rightsNanyang Technological University
dc.subjectDRNTU::Engineering::Computer science and engineeringen_US
dc.titleStructural modeling of LAG-3 protein and immunoglobulin domainsen_US
dc.typeFinal Year Project (FYP)en_US
dc.contributor.supervisorKwoh Chee Keongen_US
dc.contributor.schoolSchool of Computer Engineeringen_US
dc.description.degreeBachelor of Engineering (Computer Engineering)en_US
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Appears in Collections:SCSE Student Reports (FYP/IA/PA/PI)
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