Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/59763
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dc.contributor.authorNg, Boon Kiat
dc.date.accessioned2014-05-14T03:24:02Z
dc.date.available2014-05-14T03:24:02Z
dc.date.copyright2014en_US
dc.date.issued2014
dc.identifier.urihttp://hdl.handle.net/10356/59763
dc.description.abstractEmbryonic Stem Cells (ESCs) hold great therapeutic potential due to their self-renewal and pluripotent characteristics. The polyamine regulators adenosylmethionine decarboxylase 1 (AMD1) and ornithine decarboxylase 1 (ODC1) were shown to promote ESC self-renewal. In the absence of leukemia inhibitory factor (LIF), overexpression of Amd1 in ESCs could promote ESC self-renewal. Microarray was previously performed and various genes were found to be downregulated upon LIF withdrawal and rescued upon Amd1 overexpression. Nine of such genes were chosen to be investigated as potential polyamine targets that could promote ESC self-renewal. qRT-PCR primers for five candidates were successfully designed and qRT-PCR was performed to validate the microarray results. Subsequently, aquaporin 3 (Aqp3), KH domain containing 3 (Khdc3), and zinc finger matrin type 4 (Zmat4) variant 1 and 2 were chosen for cloning but the high GC content of Khdc3 impeded the cloning process. Aqp3 and Zmat4 were overexpressed and Aqp3 was knocked down in mouse ESCs. Zmat4 did not have self-positive feedback regulation. Although transfection was successful in the overexpression and knockdown studies, the ESCs did not respond to the positive controls, possibly due to cell death and cell density respectively. Therefore, the effect of Aqp3 and Zmat4 on ESC self-renewal was inconclusive.en_US
dc.format.extent57 p.en_US
dc.language.isoenen_US
dc.rightsNanyang Technological University
dc.subjectDRNTU::Science::Biological sciencesen_US
dc.titleIdentification of new polyamine targets as stem cell regulatorsen_US
dc.typeFinal Year Project (FYP)en_US
dc.contributor.schoolSchool of Biological Sciencesen_US
dc.description.degreeBachelor of Science in Biological Sciencesen_US
dc.contributor.organizationA*STAR Institute of Medical Biologyen_US
dc.contributor.supervisor2Leah A. Vardyen_US
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Appears in Collections:SBS Student Reports (FYP/IA/PA/PI)
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