Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/60630
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dc.contributor.authorThian, Adrian Zhang Yong
dc.date.accessioned2014-05-29T03:05:57Z
dc.date.available2014-05-29T03:05:57Z
dc.date.copyright2014en_US
dc.date.issued2014
dc.identifier.urihttp://hdl.handle.net/10356/60630
dc.description.abstractAs recreational activities in Singapore are on the rise, swimming is, no doubt one of the favourite recreational activities one can enjoy in our tropical climate. However, infected recreational waters can cause pool users to be exposed to etiological agents, thereby causing waterborne outbreaks and diseases. To date, there are minimal surveillance reports in Singapore which can provide insights as to what microbiological hazards may be present in the pool. Thus, in this study, we considered a two-pronged approach to determine the potential bacterial pathogens. First, culture-dependent method accounted for medical and diagnostic aspects; second, culture-independent molecular metagenomic methods were employed as to account for non-culturable and fastidious organisms. 16S rRNA gene-based studies and metagenomic studies (using Illumina and 454-pyrosequencing) were used in this study. Results showed that gram-negative beta-lactamase bacterium, Aeromonas and Chromobacterium, were found in the pool backwash units. Draft genomes of Aeromonas obtained from Illumina sequencer also provided a high discriminatory power at the species level. Gram-negative bacteria are usually the causative agents that infect aquatic environments. Thus, these results suggest the presence of etiological agents in the pool and the need to contribute pathological insights in recreational waters in Singapore.en_US
dc.format.extent44 p.en_US
dc.language.isoenen_US
dc.rightsNanyang Technological University
dc.subjectDRNTU::Science::Biological sciences::Molecular biologyen_US
dc.subjectDRNTU::Science::Biological sciences::Microbiologyen_US
dc.titleDetection of pathogens in swimming poolen_US
dc.typeFinal Year Project (FYP)en_US
dc.contributor.schoolSchool of Biological Sciencesen_US
dc.description.degreeBachelor of Science in Biological Sciencesen_US
dc.contributor.organizationSingapore Centre for Environmental Life Sciences Engineeringen_US
dc.contributor.supervisor2Yang Liangen_US
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Appears in Collections:SBS Student Reports (FYP/IA/PA/PI)
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