Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/60632
Title: Determination of the life span of inflammatory dendritic cells
Authors: Tay, Yong Long
Keywords: DRNTU::Science::Biological sciences::Molecular biology
Issue Date: 2014
Abstract: Dendritic cells (DCs) are highly efficient antigens presenting cells (APCs) which are responsible in processing and presenting antigens to naïve T-cells thereby activating the adaptive immune response. Being recognized as an essential mediator between the innate and adaptive immunity during inflammation until recently, DC is found to have a major role in intestinal immune responses. With its ability to orchestrate between tolerance and immunity in the gut, DC deregulations contribute to inflammatory bowel diseases such as Crohn’s disease and ulcerative colitis. A novel DC subset, Plet-1+ DCs, discovered by Ruedl’s laboratory have been under extensive characterization to understand its role during inflammation. Due to the lack of a proper Plet-1 knockout mice model previously, cell ablation study of Plet-1+ DCs was not possible. By taking advantage of the newly generated chimeric mice, in this study, we aim to elucidate the turnover rate of Plet-1+ DC in both steady state and inflammatory condition. Our results established the turnover rate for steady state and DSS-induced colitis to be 5 and 3 days respectively. Furthermore, we revealed the localization of Plet-1+ DC in PP and colon using immunohistological staining consistent to the result in the previous studies. More importantly, we demonstrated the presence of Plet-1+ DCs in brain of P.berghei infected mice which open up a new disease model for the characterization of Plet-1+ DCs. In conclusion, our findings on the turnover rate of Plet-1+ DC can be used in future studies to maintain the ablation of this population for its characterization during steady state and DSS-induced inflammation.
URI: http://hdl.handle.net/10356/60632
Rights: Nanyang Technological University
Fulltext Permission: restricted
Fulltext Availability: With Fulltext
Appears in Collections:SBS Student Reports (FYP/IA/PA/PI)

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