Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/61591
Title: Investigating the role of DDX5/p68 RNA helicase in adipogenesis
Authors: Lim, Nicole Si Xiu
Keywords: DRNTU::Science::Biological sciences::Molecular biology
Issue Date: 2014
Abstract: Adipocytes are now recognized to be an essential regulator of whole-body homeostasis. The selective loss of adipose tissue results in a heterogeneous acquired or inherited disorder called lipodystrophy. Mutations in the Lamin A (LMNA) gene are known to cause Dunnigan-type familial partial lipodystrophy (FPLD). Dead Box Protein 5 (DDX5)/p68 is a RNA helicase protein previously described as being important in early stages of adipogenesis. Here, the role of DDX5 in adipogenesis is characterised in more detail and its potential role as an interactor with LMNA is also examined. The protein expression of DDX5 in two different pre-adipocyte cell lines, 3T3-L1 and C3H10T/12, were highest on day two of adipogenic differentiation. The knockdown of DDX5 in differentiated 3T3-L1 cells impair adipogenesis in the early stages and results in significant decreased triglycerides accumulation. The mRNA expression of key adipogenic factor PPARγ and several other adipogenic factors were also reduced. Immunofluorescence and immunoprecipitation data suggest that DDX5 co-localizes with the lamina in 3T3-L1 cells and binds to lipodystrophic mutants of Lamin A. These results are consistent with DDX5 being crucial for adipogenesis, particularly in the early stages. Taken together, these results provide insights into the potential molecular mechanisms that underlie lipodystrophy caused by mutations in LMNA.
URI: http://hdl.handle.net/10356/61591
Rights: Nanyang Technological University
Fulltext Permission: restricted
Fulltext Availability: With Fulltext
Appears in Collections:SBS Student Reports (FYP/IA/PA/PI)

Files in This Item:
File Description SizeFormat 
FYP Thesis Final Submit.pdf
  Restricted Access
1.66 MBAdobe PDFView/Open

Page view(s) 50

204
checked on Oct 29, 2020

Download(s) 50

7
checked on Oct 29, 2020

Google ScholarTM

Check

Items in DR-NTU are protected by copyright, with all rights reserved, unless otherwise indicated.