Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/61725
Full metadata record
DC FieldValueLanguage
dc.contributor.authorHeng, Fiona Ya Wen-
dc.date.accessioned2014-08-27T01:54:30Z-
dc.date.available2014-08-27T01:54:30Z-
dc.date.copyright2014en_US
dc.date.issued2014-
dc.identifier.urihttp://hdl.handle.net/10356/61725-
dc.description.abstractCysteine rich peptides are an important area in pharmaceutical research as they possess potential therapeutic uses due to their high conformational stability. Hence, a small scale extraction of herbs was conducted in attempt to identify herbs which are abundant in CRPs. Each herb produced a unique spectrum which is useful for classification and identification. Another aim of this study is to isolate, purify and characterize peptides found in Lycium barbarum, a commonly used traditional Chinese herb. In this study, we have successfully isolated and purify 3 peptides unique to Lycium barbarum, with masses (Da) of 3838, 3992 and 4153 from our sample using HPLC. Lycium barbarum was tested on HUVEC cell line in a cell proliferation assay, in which it was discovered to be anti-proliferative at higher concentrations. It may be a potential biologic for cancer treatment that prevents tumour progression due to its anti-angiogenic effect. A peptide that we had isolated from Lycium barbarum was found to be 70% homologous to potato Carboxypeptidase inhibitor which provided insights for functional studies. From the Carboxypeptidase inhibition assay, we discovered that the peptide also has Carboxypeptidase inhibition activity. Hence, it may have great potential for future development into an oral bioactive biologic.en_US
dc.format.extent43 p.en_US
dc.language.isoenen_US
dc.rightsNanyang Technological University-
dc.subjectDRNTU::Scienceen_US
dc.titleDiscovery and characterization of Lycium barbarum peptidesen_US
dc.typeFinal Year Project (FYP)en_US
dc.contributor.supervisorJames P. Tamen_US
dc.contributor.schoolSchool of Biological Sciences-
dc.description.degreeBachelor of Science in Biomedical Sciencesen_US
item.fulltextWith Fulltext-
item.grantfulltextrestricted-
Appears in Collections:SBS Student Reports (FYP/IA/PA/PI)
Files in This Item:
File Description SizeFormat 
FYP -Fiona.pdf
  Restricted Access
1.07 MBAdobe PDFView/Open

Page view(s) 20

628
Updated on Jul 14, 2024

Download(s) 50

34
Updated on Jul 14, 2024

Google ScholarTM

Check

Items in DR-NTU are protected by copyright, with all rights reserved, unless otherwise indicated.