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https://hdl.handle.net/10356/62943
Title: | Hydrogen/deuterium exchange mass spectrometry reveals distinct mechanisms of viral RNA recognition and activation by RIG-I like receptors | Authors: | Zheng, Jie | Keywords: | DRNTU::Science::Biological sciences::Biophysics | Issue Date: | 2015 | Source: | Zheng, J. (2015). Hydrogen/deuterium exchange mass spectrometry reveals distinct mechanisms of viral RNA recognition and activation by RIG-I like receptors. Doctoral thesis, Nanyang Technological University, Singapore. | Abstract: | RIG-I like receptors (RLRs) are the major intracellular immune receptors that recognize viral RNA species, undergo a series of conformational transitions and activate the interferon-mediated antiviral response. However, full-length RLRs resist crystallographic efforts and a molecular description of their activation pathways remains hypothetical. Here we employ Hydrogen/deuterium exchange coupled with mass spectrometry (HDX-MS) to probe the apo states of RLRs and to dissect the molecular details with respect to RNA recognition, ATP hydrolysis and CARDs activation. Our data provide a high-resolution orthogonal view of RLR activation in solutions and offer new evidence for the molecular mechanism of RLR activation. | URI: | https://hdl.handle.net/10356/62943 | DOI: | 10.32657/10356/62943 | Fulltext Permission: | open | Fulltext Availability: | With Fulltext |
Appears in Collections: | SBS Theses |
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File | Description | Size | Format | |
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Zheng Jie Thesis .pdf | 41.72 MB | Adobe PDF | ![]() View/Open |
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