Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/62943
Title: Hydrogen/deuterium exchange mass spectrometry reveals distinct mechanisms of viral RNA recognition and activation by RIG-I like receptors
Authors: Zheng, Jie
Keywords: DRNTU::Science::Biological sciences::Biophysics
Issue Date: 2015
Source: Zheng, J. (2015). Hydrogen/deuterium exchange mass spectrometry reveals distinct mechanisms of viral RNA recognition and activation by RIG-I like receptors. Doctoral thesis, Nanyang Technological University, Singapore.
Abstract: RIG-I like receptors (RLRs) are the major intracellular immune receptors that recognize viral RNA species, undergo a series of conformational transitions and activate the interferon-mediated antiviral response. However, full-length RLRs resist crystallographic efforts and a molecular description of their activation pathways remains hypothetical. Here we employ Hydrogen/deuterium exchange coupled with mass spectrometry (HDX-MS) to probe the apo states of RLRs and to dissect the molecular details with respect to RNA recognition, ATP hydrolysis and CARDs activation. Our data provide a high-resolution orthogonal view of RLR activation in solutions and offer new evidence for the molecular mechanism of RLR activation.
URI: https://hdl.handle.net/10356/62943
DOI: 10.32657/10356/62943
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:SBS Theses

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