dc.contributor.authorZheng, Jie
dc.date.accessioned2015-05-04T04:21:28Z
dc.date.accessioned2017-07-23T08:42:54Z
dc.date.available2015-05-04T04:21:28Z
dc.date.available2017-07-23T08:42:54Z
dc.date.copyright2015en_US
dc.date.issued2015
dc.identifier.citationZheng, J. (2015). Hydrogen/deuterium exchange mass spectrometry reveals distinct mechanisms of viral RNA recognition and activation by RIG-I like receptors. Doctoral thesis, Nanyang Technological University, Singapore.
dc.identifier.urihttp://hdl.handle.net/10356/62943
dc.description.abstractRIG-I like receptors (RLRs) are the major intracellular immune receptors that recognize viral RNA species, undergo a series of conformational transitions and activate the interferon-mediated antiviral response. However, full-length RLRs resist crystallographic efforts and a molecular description of their activation pathways remains hypothetical. Here we employ Hydrogen/deuterium exchange coupled with mass spectrometry (HDX-MS) to probe the apo states of RLRs and to dissect the molecular details with respect to RNA recognition, ATP hydrolysis and CARDs activation. Our data provide a high-resolution orthogonal view of RLR activation in solutions and offer new evidence for the molecular mechanism of RLR activation.en_US
dc.format.extent178 p.en_US
dc.language.isoenen_US
dc.subjectDRNTU::Science::Biological sciences::Biophysicsen_US
dc.titleHydrogen/deuterium exchange mass spectrometry reveals distinct mechanisms of viral RNA recognition and activation by RIG-I like receptorsen_US
dc.typeThesis
dc.contributor.researchBioSciences Research Centreen_US
dc.contributor.schoolSchool of Biological Sciencesen_US
dc.contributor.supervisorTang Kai
dc.contributor.supervisorLiu Chuan Faen_US
dc.description.degreeDOCTOR OF PHILOSOPHY (SBS)en_US


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