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Title: Study of Xist-mediated gene silencing using iXist
Authors: Tan, Jin Da
Keywords: DRNTU::Science::Biological sciences::Genetics
Issue Date: 2015
Abstract: X Chromosome Inactivation (XCI) is an example of dosage compensation in mammals in which one of the X chromosomes in females is inactivated to balance the gene dosage between males and females. This process is dependent on a long non-coding RNA (lncRNA) called Xist. Previous report showed that Xist cDNA was sufficient to cause gene silencing in mouse cells. In this study, we used an inducible-expression system to compare the gene silencing capability of full length Xist and Xist cDNA directly. Both transgenes showed efficient gene silencing effect but full length Xist showed a slight but significant higher gene silencing capacity as compared to that of Xist cDNA. This shows that RNA splicing or the presence of introns may play a role in Xist-mediated gene silencing. Different Xist mutants such as Xist cDNA with insertion of intron 1 or 4 were then generated to determine the specific roles of RNA splicing and presence of introns.
Rights: Nanyang Technological University
Fulltext Permission: restricted
Fulltext Availability: With Fulltext
Appears in Collections:SBS Student Reports (FYP/IA/PA/PI)

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