Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/63953
Title: Roles of CXCL1 and CXCL2 on neutrophils during immune complex-mediated inflammation
Authors: Li, Jackson Liangyao
Keywords: DRNTU::Science::Biological sciences::Microbiology::Immunology
DRNTU::Science::Biological sciences::Molecular biology
Issue Date: 2014
Abstract: CXCR2 is a critical regulator of neutrophil migration, and has been proposed to play an important role in immune complex-mediated inflammation. In the mouse, major pro-inflammatory CXCR2 ligands include CXCL1/KC/GRO-α and CXCL2/MIP-2a/GRO-β, but their specific individual roles on neutrophils have remained largely unclear. We developed an intravital multiphoton microscopy model of the reverse passive Arthus reaction in the mouse ear skin that allowed us to image neutrophil interactions with immune complexes. In combination with other in vitro and in vivo assays, we discovered that both CXCL1 and CXCL2 played very similar roles on neutrophil activation, but CXCL2 was the major chemokine produced by the neutrophils in feed-forward and autocrine loops, whereas CXCL1, produced by sentinel cell types such as macrophages and endothelial cells, could enhance neutrophil CXCL2 production. Our study reveals the complexity of CXCR2 ligand crosstalk during immune complex-mediated inflammation, and elucidates an intriguing mechanism of neutrophil biology. Keywords: neutrophil, immune complex, inflammation, CXCR2, CXCL1, CXCL2, in vivo imaging, multiphoton microscopy
URI: http://hdl.handle.net/10356/63953
Fulltext Permission: restricted
Fulltext Availability: With Fulltext
Appears in Collections:SBS Theses

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LI LIANGYAO JACKSON Full set thesis_Amended.pdf
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Main Article7.65 MBAdobe PDFView/Open
Supplementary video 1_Speckling comparison.mov
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Supplementary Video 111.91 MBVideo QuicktimeView/Open
Supplementary video 2_RPA reaction.mov
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Supplementary Video 214.51 MBVideo QuicktimeView/Open
Supplementary video 3_RPA reaction zoomed in.mov
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Supplementary Video 35.86 MBVideo QuicktimeView/Open
Supplementary video 4_Neutrophil depletion RPA.mov
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Supplementary Video 42.95 MBVideo QuicktimeView/Open
Supplementary video 5_RPA with AntiCXCL1.mov
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Supplementary Video 516.99 MBVideo QuicktimeView/Open
Supplementary video 6_RPA with AntiCXCL2.mov
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Supplementary Video 614.72 MBVideo QuicktimeView/Open
Supplementary video 7_IC removal RPA CD11a KO.mov
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Supplementary Video 71.64 MBVideo QuicktimeView/Open

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