Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/64402
Title: Conjugation of polyethylene glycol to ferritin nanocage
Authors: Shuhaida Mohamed Salleh
Keywords: DRNTU::Engineering::Bioengineering
Issue Date: 2015
Abstract: Research has shown that protein cages have potential applications such as drug delivery vehicle and MRI contrast agents. However, their application is hampered by their immunogenicity. In this project, a polymer is conjugated to the protein cage to reduce immunogenic response. Archaeoglobus fulgidus ferritin mutant (AfFtn-AA) with 24 subunits that self-assemble to protein cage in the presence of iron ions was used. The amino acid glutamic acid at site 94 was substituted with cysteine residue, providing a thiol group at each subunit. The protein cage was then conjugated to polyethylene glycol (PEG) molecules of molecular weights 2, 5 and 10 kDa and a 4-arm PEG of molecular weight 20 kDa. Two conditions were tested to optimize the conjugation reaction and the modified protein cage was characterized by dynamic light scattering (DLS), matrix assisted laser desorption/ionization time-of-flight (MALDI TOF) mass spectrometry and transmission electron microscopy (TEM). The conjugation reaction with different sizes of PEG molecules led to a shift in peaks in the DLS and MALDI TOF analysis gave the profile of each protein-polymer conjugate. The conjugation of the protein cage to PEG implies that the protocol is effective and can be used as a platform for future work as carrier for drug delivery.
URI: http://hdl.handle.net/10356/64402
Fulltext Permission: restricted
Fulltext Availability: With Fulltext
Appears in Collections:SCBE Theses

Files in This Item:
File Description SizeFormat 
ShuhaidaBinteMohamedSalleh15.pdf
  Restricted Access
1.89 MBAdobe PDFView/Open

Google ScholarTM

Check

Items in DR-NTU are protected by copyright, with all rights reserved, unless otherwise indicated.