Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/64625
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dc.contributor.authorLim, Ken Choong
dc.date.accessioned2015-05-29T01:56:04Z
dc.date.available2015-05-29T01:56:04Z
dc.date.copyright2015en_US
dc.date.issued2015
dc.identifier.urihttp://hdl.handle.net/10356/64625
dc.description.abstractThe development and commercial availability of ultrafast femtosecond lasers with high peak power enabled strong optical fields to be generated in a straightforward manner. Since then, nonlinear optics and nonlinear optical imaging has proven to be an effective tool for investigation and analysis of various samples in biology, microelectronics and other domains. In this project, a Nonlinear Laser Scanning Microscope (NLSM) is setup, aligned and calibrated in order to image various samples of organic and inorganic origins. Using the NLSM, nonlinear optical images from SLS, Native and FLS collagen structures were obtained and correlated with their linear optical signals to obtain nonlinear optical signatures of the collagen structures. In addition, nonuniform SHG signal distribution from native collagen fibers were observed, an effect plausibly due to SHG geometric resonance. Uniformly distributed TPEF signals were detected in SLS and FLS collagen structures, providing preliminary evidence for the uniform incorporation of glycoprotein and ATP in the ultrastructures of FLS and SLS collagen respectively. Furthermore, the NLSM was used to perform 3D tomographic imaging on electroluminescent displays printed on a plastic substrate. Image blurring and distortions were discovered to be caused by birefringence induced astigmatism originating from the plastic substrate. Simulations were performed to correlate the experimentally observed phenomena to a proposed analytical model and methods to reduce image distortions due to astigmatism are briefly discussed. Lastly, in order to further extend the capabilities of the NLSM, a methodology to batch stitch images obtained using an NLSM was developed to achieve wide field-of-view 3D tomography.en_US
dc.format.extent78 p.en_US
dc.language.isoenen_US
dc.rightsNanyang Technological University
dc.subjectDRNTU::Engineering::Electrical and electronic engineering::Optics, optoelectronics, photonicsen_US
dc.subjectDRNTU::Engineering::Bioengineeringen_US
dc.subjectDRNTU::Engineering::Electrical and electronic engineering::Microelectronicsen_US
dc.subjectDRNTU::Science::Physics::Optics and lighten_US
dc.titleNonlinear optical imaging investigations at the micro-scaleen_US
dc.typeFinal Year Project (FYP)en_US
dc.contributor.supervisorLi Hao
dc.contributor.supervisorWang Qijieen_US
dc.contributor.schoolSchool of Electrical and Electronic Engineeringen_US
dc.description.degreeBachelor of Engineeringen_US
dc.contributor.organizationA*STAR SIMTechen_US
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Appears in Collections:EEE Student Reports (FYP/IA/PA/PI)
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