Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/65096
Title: Labeling of pseudomonas aeruginosa with quantum dots
Authors: Tan Nurul Wahidah Abdul Hadi
Keywords: DRNTU::Engineering::Bioengineering
Issue Date: 2015
Abstract: Quantum Dots (QDs) technique has been one of the leading methods that gain recognition in the recent years. In order to leverage on this unique properties QDs possess, and the fatality of Pseudomonas Aeruginosa infection has been on the increasing trend, P.aeruginosa was experimented to explore the behavior on how well it can attached to QDs in its bacteria cells. This report aims to successfully labeled P.aeruginosa with QDs for body tracking using three different methods of assessment: flow cytometry, epifluorescence microscopes and fluorescence spectrophotometry. The characteristics of P.aeruginosa are also discovered, and the challenges faced examined. The attempt of labeling Outer Membrane Vesicles (OMVs) is also included in the experiment at the later part and was analyzed using fluorescence spectrophotometer. There is room for improvement for future studies in terms of the process involved for each experiment and apparatus used to maximize utility so that the results deduced are clearer.
URI: http://hdl.handle.net/10356/65096
Rights: Nanyang Technological University
Fulltext Permission: restricted
Fulltext Availability: With Fulltext
Appears in Collections:SCBE Student Reports (FYP/IA/PA/PI)

Files in This Item:
File Description SizeFormat 
FYP report TAN NURUL WAHIDAH U1121416F.pdf
  Restricted Access
13.95 MBAdobe PDFView/Open

Google ScholarTM

Check

Items in DR-NTU are protected by copyright, with all rights reserved, unless otherwise indicated.