Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/65101
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dc.contributor.authorGuo, Fangfang
dc.date.accessioned2015-06-15T02:01:25Z
dc.date.available2015-06-15T02:01:25Z
dc.date.copyright2015en_US
dc.date.issued2015
dc.identifier.urihttp://hdl.handle.net/10356/65101
dc.description.abstractCyclic mechanical loading on Mesenchymal Stem Cells (MSCs) has resulted in increased expression of early osteogenic markers as well as increased mineralized matrix deposition. In most of past studies, scaffolds were subjected to the cyclic loading immediately after seeding or a few days after static culture. However, sufficient cell numbers and stable attachment of cells could enhance better mechanotransduction and accelerate bone formation. The aim of the study was to investigate the osteogenic responses of well proliferated human fetal mesenchymal stem cells (hfMSC) seeded scaffolds to cyclic compression loading, using biaxial rotating bioreactor to proliferate the cells first and the mini static bioreactor to induce appropriate load and strain. Preliminary study was done to investigate the most effective strain that enhances bone formation of hfMSCs. Preliminary study demonstrated that cyclic compression at physiological related strain (2,200 μɛ) best enhances osteogenesis and proliferation of hfMSCs, and this strain was applied in the second study. Results in the second study showed that proliferation was inhibited in the loaded group in a short-term culture (7 days) while enhanced in long-term (14 days). ALP activity was enhanced (1.35 fold) in cyclic loaded group at day 14, which in turn result in promoted mineralization. Conclusion can be drawn that cyclic compression enhances the osteogenesis and mineralization on well proliferated cellular scaffolds.en_US
dc.format.extent56 p.en_US
dc.language.isoenen_US
dc.rightsNanyang Technological University
dc.subjectDRNTU::Engineering::Bioengineeringen_US
dc.titleCyclic compression of mesenchymal stem cells loaded scaffolds for bone tissue engineeringen_US
dc.typeFinal Year Project (FYP)en_US
dc.contributor.supervisorTeoh Swee Hinen_US
dc.contributor.schoolSchool of Chemical and Biomedical Engineeringen_US
dc.description.degreeBachelor of Engineering (Chemical and Biomolecular Engineering)en_US
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Appears in Collections:SCBE Student Reports (FYP/IA/PA/PI)
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