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Title: Confined biofilm culture and flow in microfluidic channel
Authors: Lim, Chun Ping
Keywords: DRNTU::Engineering::Mechanical engineering::Fluid mechanics
DRNTU::Science::Biological sciences::Microbiology::Bacteria
Issue Date: 2015
Source: Lim, C. P. (2015). Confined biofilm culture and flow in microfluidic channel. Doctoral thesis, Nanyang Technological University, Singapore.
Abstract: A biofilm is an aggregate of bacteria held together by an extracellular polymeric matrix on an interface. A biofilm residing on a liquid-solid interface is significantly influenced by the hydrodynamic environment. Although various microfluidic flow cells have been developed to study biofilms under well-defined flow conditions, the control of biofilm growth remains a challenge. Nutrient zoning was developed to achieve biofilm growth confinement and was demonstrated in two microfluidic flow cells, namely a T-shaped flow cell (T flow cell) and a multiplexed flow cell, using two-phase immiscible fluid flow. Complete confinement of biofilm growth was achieved which provided a reference surface free of biofilm in the same channel to facilitate post-experiment analysis. Microfluidics generally operates in the laminar flow regime. However, chaotic and turbulent flows can have significant effects on the structure and function of biofilms. Thus, a platform for generating viscoelastic chaotic flow in micro-channels was developed. Chaotic flows of polymer solutions were generated in an H-shaped micro-channel (H-micro-channel) and the mechanisms of their generation were studied. Furthermore, characteristic and maps of the chaotic flows were constructed which can be employed to generate a defined chaotic flow to study biofilm systematically.
Fulltext Permission: restricted
Fulltext Availability: With Fulltext
Appears in Collections:MAE Theses

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