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Title: | Confined biofilm culture and flow in microfluidic channel | Authors: | Lim, Chun Ping | Keywords: | DRNTU::Engineering::Mechanical engineering::Fluid mechanics DRNTU::Science::Biological sciences::Microbiology::Bacteria |
Issue Date: | 2015 | Source: | Lim, C. P. (2015). Confined biofilm culture and flow in microfluidic channel. Doctoral thesis, Nanyang Technological University, Singapore. | Abstract: | A biofilm is an aggregate of bacteria held together by an extracellular polymeric matrix on an interface. A biofilm residing on a liquid-solid interface is significantly influenced by the hydrodynamic environment. Although various microfluidic flow cells have been developed to study biofilms under well-defined flow conditions, the control of biofilm growth remains a challenge. Nutrient zoning was developed to achieve biofilm growth confinement and was demonstrated in two microfluidic flow cells, namely a T-shaped flow cell (T flow cell) and a multiplexed flow cell, using two-phase immiscible fluid flow. Complete confinement of biofilm growth was achieved which provided a reference surface free of biofilm in the same channel to facilitate post-experiment analysis. Microfluidics generally operates in the laminar flow regime. However, chaotic and turbulent flows can have significant effects on the structure and function of biofilms. Thus, a platform for generating viscoelastic chaotic flow in micro-channels was developed. Chaotic flows of polymer solutions were generated in an H-shaped micro-channel (H-micro-channel) and the mechanisms of their generation were studied. Furthermore, characteristic and maps of the chaotic flows were constructed which can be employed to generate a defined chaotic flow to study biofilm systematically. | URI: | http://hdl.handle.net/10356/65306 | Schools: | School of Mechanical and Aerospace Engineering | Organisations: | Singapore-MIT Alliance for Research and Technology (BioSystems and Micromechanics Inter-Disciplinary Research Group) | Research Centres: | Singapore-MIT Alliance Programme | Fulltext Permission: | restricted | Fulltext Availability: | With Fulltext |
Appears in Collections: | MAE Theses |
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2015_Confined Biofilm Culture and Flow in Microfluidic Channel.pdf Restricted Access | 6.19 MB | Adobe PDF | View/Open |
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