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https://hdl.handle.net/10356/65663
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DC Field | Value | Language |
---|---|---|
dc.contributor.author | Toh, Desiree-Faye Kaixin | |
dc.date.accessioned | 2015-12-03T08:39:06Z | |
dc.date.available | 2015-12-03T08:39:06Z | |
dc.date.copyright | 2015 | en_US |
dc.date.issued | 2015 | |
dc.identifier.citation | Toh, D.-F. K. (2015). Incorporation of modified bases into triplex-forming peptide nucleic acids for the recognition of a C-G pyrimidine-purine inversion site of an RNA duplex. Master's thesis, Nanyang Technological University, Singapore. | |
dc.identifier.uri | http://hdl.handle.net/10356/65663 | |
dc.description.abstract | Peptide nucleic acid (PNA), an analogue of DNA, can enhance the recognition of specific sequences in RNA duplexes and may be useful therapeutic reagents. In this study, a PNA monomer (Q) containing a N4-(2-guanidoethyl)-5-methylcytosine base was synthesized and incorporated into 8-mer PNAs. Together with previously reported thio-pseudoisocytosine (L) monomer, the non-denaturing polyacrylamide gel electrophoresis results suggest that the PNA is able to selectively target RNA hairpins containing a pyrimidine-purine (C-G) inversion with high binding affinity. The PNA does not bind to DNA hairpins. Thermal melting experiments indicate that PNA containing Q monomer is not able to bind to single-stranded RNA. | en_US |
dc.format.extent | 52 p. | en_US |
dc.language.iso | en | en_US |
dc.subject | DRNTU::Science::Chemistry::Biochemistry | en_US |
dc.title | Incorporation of modified bases into triplex-forming peptide nucleic acids for the recognition of a C-G pyrimidine-purine inversion site of an RNA duplex | en_US |
dc.type | Thesis | |
dc.contributor.supervisor | Chen Gang | en_US |
dc.contributor.school | School of Physical and Mathematical Sciences | en_US |
dc.description.degree | Master of Science | en_US |
item.fulltext | With Fulltext | - |
item.grantfulltext | restricted | - |
Appears in Collections: | SPMS Theses |
Files in This Item:
File | Description | Size | Format | |
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Msc Thesis_Desiree-Faye Toh (G1303248K).pdf Restricted Access | Msc Thesis | 3.08 MB | Adobe PDF | View/Open |
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