Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/6574
Title: Molecular studies of the replication mechanisms of coronavirus
Authors: Wang, Jibin
Keywords: DRNTU::Science::Biological sciences
Issue Date: 2007
Source: Wang, J. B. (2007). Molecular studies of the replication mechanisms of coronavirus. Doctoral thesis, Nanyang Technological University, Singapore.
Abstract: Coronavirus M protein is an essential component of virions and plays pivotal roles in virion assembly, budding and maturation. The M protein is integrated into the viral envelope with three transmembrane domains flanked by a short amino-terminal ectodomain and a large carboxy-terminal endodomain. To understand the cellular factors that may be involved in virion assembly, budding and maturation processes, the M protein from coronavirus infectious bronchitis virus (IBV) was used as a bait in a yeast two-hybrid screen, resulting in the identification of -Actin as a potentially interacting partner. This interaction was subsequent confirmed by coimmunoprecipitation and immunofluorescence in mammalian cells, and mutation of amino acids A159 and K160 in the M protein abolished the interaction.
URI: https://hdl.handle.net/10356/6574
DOI: 10.32657/10356/6574
Rights: Nanyang Technological University
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:SBS Theses

Files in This Item:
File Description SizeFormat 
SBS-THESES_20.pdf2.58 MBAdobe PDFThumbnail
View/Open

Google ScholarTM

Check

Altmetric


Plumx

Items in DR-NTU are protected by copyright, with all rights reserved, unless otherwise indicated.