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https://hdl.handle.net/10356/6574
Title: | Molecular studies of the replication mechanisms of coronavirus | Authors: | Wang, Jibin | Keywords: | DRNTU::Science::Biological sciences | Issue Date: | 2007 | Source: | Wang, J. B. (2007). Molecular studies of the replication mechanisms of coronavirus. Doctoral thesis, Nanyang Technological University, Singapore. | Abstract: | Coronavirus M protein is an essential component of virions and plays pivotal roles in virion assembly, budding and maturation. The M protein is integrated into the viral envelope with three transmembrane domains flanked by a short amino-terminal ectodomain and a large carboxy-terminal endodomain. To understand the cellular factors that may be involved in virion assembly, budding and maturation processes, the M protein from coronavirus infectious bronchitis virus (IBV) was used as a bait in a yeast two-hybrid screen, resulting in the identification of -Actin as a potentially interacting partner. This interaction was subsequent confirmed by coimmunoprecipitation and immunofluorescence in mammalian cells, and mutation of amino acids A159 and K160 in the M protein abolished the interaction. | URI: | https://hdl.handle.net/10356/6574 | DOI: | 10.32657/10356/6574 | Schools: | School of Biological Sciences | Rights: | Nanyang Technological University | Fulltext Permission: | open | Fulltext Availability: | With Fulltext |
Appears in Collections: | SBS Theses |
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SBS-THESES_20.pdf | 2.58 MB | Adobe PDF | View/Open |
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