Please use this identifier to cite or link to this item:
https://hdl.handle.net/10356/67318
Title: | Role of WIP in metastasis and study of transcriptional regulation of N-Wasp | Authors: | Amrita S Salvi | Keywords: | DRNTU::Science::Biological sciences | Issue Date: | 2016 | Source: | Amrita S Salvi. (2016). Role of WIP in metastasis and study of transcriptional regulation of N-Wasp. Doctoral thesis, Nanyang Technological University, Singapore. | Abstract: | Tumor cell migration and invasion involves actin cytoskeleton reorganization, which is regulated by N-WASP (Neural-Wiskott Aldrich Syndrome Protein) and its interacting proteins such as WASP interacting protein (WIP). Expression of WIP was found to be higher in metastatic cancer cell line compared to its non-metastatic parental cell line. Overexpression of WIP was found to enhance the proliferative, migratory and invasive ability as well as confer anchorage independent growth properties in A549 lung carcinoma cells indicating a pro-metastatic function of WIP. Expression of N-WASP is enhanced in cells undergoing epithelial mesenchymal transition induced by hypoxia. In order to identify mechanism responsible for the differential expression of N-WASP, the N-WASP promoter was characterized, which led to the identification of HRE (Hypoxia Response element) a regulatory region in N-WASP promoter. Our results suggest that transcription factor HiF1α regulates N-WASP expression in promoting metastasis under hypoxic conditions. | URI: | https://hdl.handle.net/10356/67318 | DOI: | 10.32657/10356/67318 | Schools: | School of Biological Sciences | Fulltext Permission: | open | Fulltext Availability: | With Fulltext |
Appears in Collections: | SBS Theses |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
Amrita Salvi_Thesis.pdf | 6.6 MB | Adobe PDF | View/Open |
Page view(s)
389
Updated on Mar 18, 2024
Download(s) 20
252
Updated on Mar 18, 2024
Google ScholarTM
Check
Altmetric
Items in DR-NTU are protected by copyright, with all rights reserved, unless otherwise indicated.