Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/68382
Title: Development of inervertebral disc with functional nucleus pulposus for clinical application
Authors: Tee, Ching Ann
Keywords: DRNTU::Engineering
Issue Date: 2016
Abstract: Lower back pain caused by degenerative disc disease are getting more common in the society. Current treatments available such as spinal fusion and artificial disc displacement are able to relieve pain but fail to restore the function of intervertebral disc (IVD) and associated with side effect. This has led to increase interest in tissue engineering of IVD which aim to regenerate tissue’s native function through scaffold, cell and/or bioactive agent. In this project, the role of nucleus pulposus chondrocyte (NPC), human mesenchymal stem cell (hMSC) and growth factors in tissue engineering of nucleus pulposus (NP) was investigated. NPC was sorted base on its physical size into smaller and bigger size population. Through gene expression analysis and F-actin staining, smaller size population was found to be able to form NP tissue construct that synthesis higher level of major NP matrix component such as aggrecan and Type II Collagen. Expansion of isolated patient’s cell has been the prerequisite for tissue engineering but it is associated with de-differentiation. Therefore, the role of hMSC and effect of encapsulated growth factors in forming NP tissue construct was studied. It was found that hMSC was able to express more NP matrix when encapsulated with both transforming growth factor beta 3 (TGF-β3) and platelet-derived growth factor-BB (PDGF-BB) than each growth factor alone. The result of this project showed that cell encapsulating hydrogel has the potential to be applied clinically with the injectable property of hydrogel that allowed surgery to be carried out in minimal invasive way.
URI: http://hdl.handle.net/10356/68382
Rights: Nanyang Technological University
Fulltext Permission: restricted
Fulltext Availability: With Fulltext
Appears in Collections:SCBE Student Reports (FYP/IA/PA/PI)

Files in This Item:
File Description SizeFormat 
FYP-Report_Final.pdf
  Restricted Access
SCBE FYP Report_Tee Ching Ann2.88 MBAdobe PDFView/Open

Page view(s)

116
Updated on Jun 17, 2021

Download(s)

13
Updated on Jun 17, 2021

Google ScholarTM

Check

Items in DR-NTU are protected by copyright, with all rights reserved, unless otherwise indicated.