Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/69932
Title: Point-of-care biosensor for stroke biomarker detection
Authors: Ng, Timothy Si En
Keywords: DRNTU::Engineering
Issue Date: 2017
Abstract: There is demand for Point-of-care devices for stroke sub-type diagnostic application. Current lateral flow assays demonstrated high specificity but low sensitivity. The Stackpad biosensor is a modification to the lateral flow assays in order to achieve a more sensitive Point-of-care device. The proof of concept was done earlier for detection of bacteria. This report optimized the factors affecting the Stackpad biosensor, allowing for further developmental testing of the device with actual samples and target stroke biomarkers. It worked on improving the flow by observing ink and water flow in the device. Membrane modification procedures and the colorimetric signal were studied by comparing with both the ELISA and Stackpad testing. After optimization, the flow of the novel device was improved with the separation layer and addition of Tween20. The Stackpad biosensor also takes less than 5 minutes to achieve response. The procedure for immobilizing HRP-conjugated antibodies on the Conjugate and release pads was also optimized to achieve acceptable level of signals. TMB was also determined to be a suitable colorimetric substrate in a 1-step Point-of-care device.
URI: http://hdl.handle.net/10356/69932
Rights: Nanyang Technological University
Fulltext Permission: restricted
Fulltext Availability: With Fulltext
Appears in Collections:MSE Student Reports (FYP/IA/PA/PI)

Files in This Item:
File Description SizeFormat 
NG SI EN TIMOTHY_U1320946K_FYP REPORT.pdf
  Restricted Access
MSE FYP2.54 MBAdobe PDFView/Open

Page view(s)

211
Updated on Jun 22, 2021

Download(s) 50

43
Updated on Jun 22, 2021

Google ScholarTM

Check

Items in DR-NTU are protected by copyright, with all rights reserved, unless otherwise indicated.