Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/70613
Title: The microbiome as a target to reduce EV71 pathogenesis and epidemic potential
Authors: Mohamed Haikal Bin Mohamed Ismail
Keywords: DRNTU::Science
Issue Date: 2017
Abstract: EV71 is an enterovirus that infects the host through the gut. The gut also houses a diverse array of commensal bacteria. However, not much is understood about viral and bacterial interactions in pathogenesis. We characterised the effects of four species of bacteria on EV71 using in vitro cell model assays. The bacteria chosen were either known to have an effect on enteroviruses or form a major constituent of gut microbiome. We observed that exposure to bacteria generally increased viral replication, with Bacillus cereus significantly increasing replication in muscle cells. We also observed that Clostridium difficile significantly increased replication in epithelial cells. Experiments conducted on TLR stimulation and viral replication revealed that intracellular TLR stimulation tend to increase viral expression significantly. The results suggest that EV71 exploits cellular pathways that respond to bacterial stimulation in order to proliferate rapidly.
URI: http://hdl.handle.net/10356/70613
Rights: Nanyang Technological University
Fulltext Permission: restricted
Fulltext Availability: With Fulltext
Appears in Collections:SBS Student Reports (FYP/IA/PA/PI)

Files in This Item:
File Description SizeFormat 
FYP_Report_Mohamed_Haikal_U1340567C.pdf
  Restricted Access
Main article1.14 MBAdobe PDFView/Open

Page view(s)

118
Updated on Jun 22, 2021

Download(s)

11
Updated on Jun 22, 2021

Google ScholarTM

Check

Items in DR-NTU are protected by copyright, with all rights reserved, unless otherwise indicated.