Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/70673
Title: Factors driving intraspecific diversity in mono-species and mixed-species biofilm
Authors: Nai, Jia Wen
Keywords: DRNTU::Science
Issue Date: 2017
Abstract: Diversity has long been recognized to play an essential role in ensuring the survival of biological communities. In particular for sessile communities such as biofilms, both intraspecific (genotypic) and interspecific (species) diversities can have significant effects on the fitness of the community under unfavourable conditions such as oxidative stress and starvation. In this project, we explored the effects of nutrients (casamino acids, glucose and fumarate) and quorum sensing (QS) on the interspecific and intraspecific diversities of Pseudomonas protegens mono-species biofilms and Pseudomonas aeruginosa, P. protegens and Klebsiella pneumoniae mixed-species biofilms. Biofilms were cultivated in three-channel flow cells for 6 days and their morphotypic variants were enumerated from days 3 to 6 based on their differences in colony morphologies as compared to the wild type. Our results suggest that casamino acids promote the formation of P. protegens variants in both mono-species and mixed-species biofilms and each of the four P. protegens variants are dominant and have varying finesses in different carbon sources. Our data also demonstrated that las and rhl QS systems do not influence the production of variants.
URI: http://hdl.handle.net/10356/70673
Rights: Nanyang Technological University
Fulltext Permission: restricted
Fulltext Availability: With Fulltext
Appears in Collections:SBS Student Reports (FYP/IA/PA/PI)

Files in This Item:
File Description SizeFormat 
Nai Jia Wen_FYP Thesis_Complete.pdf
  Restricted Access
1.76 MBAdobe PDFView/Open

Page view(s)

125
Updated on Jul 27, 2021

Download(s) 50

15
Updated on Jul 27, 2021

Google ScholarTM

Check

Items in DR-NTU are protected by copyright, with all rights reserved, unless otherwise indicated.