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Title: Algae salinity tolerance enhancement mediated by interaction with bacteria
Authors: Ng, Cheng Yee
Keywords: DRNTU::Engineering::Environmental engineering
DRNTU::Science::Biological sciences::Microbiology::Microorganisms
Issue Date: 2017
Abstract: This study investigates the mutualistic relationship between Chlamydomonas reinhardtii and Comamonas testosteroni I2 when cultured in 0.7% salinity TP media. When subjected to abiotic stresses, algal-bacterial co-culture yielded better growth compared to algal monoculture, a result which was observed across three bacterial strains. Specimens in saline media displayed higher chlorophyll concentrations in co-culture than in monoculture, and when cultivated in an environment of high surface area to volume ratio than in liquid suspension. Analysis of supernatant samples extracted and filtered from 96-well microplate samples indicated the production of organic carbon by alga, implicating cross-feeding between algae and vitamin B12-producing I2. Cell imaging provided visual evidence of bacterial biofilm formation surrounding algae cells.
Rights: Nanyang Technological University
Fulltext Permission: restricted
Fulltext Availability: With Fulltext
Appears in Collections:CEE Student Reports (FYP/IA/PA/PI)

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