Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/71744
Title: Mechanical properties of tau pre-mRNA splice site hairpin and its mutants
Authors: Shi, Jiahao
Keywords: DRNTU::Science::Chemistry
Issue Date: 2017
Source: Shi, J. (2017). Mechanical properties of tau pre-mRNA splice site hairpin and its mutants. Doctoral thesis, Nanyang Technological University, Singapore.
Abstract: Understanding the behavior of RNA during the translation and other processes becomes more and more important today since knowledge of biological functions of RNA has increased rapidly. In the last few decades the development of technologies makes the single molecules manipulation much more convenient and precise, which makes it possible to investigate the relationship between these bio-functions and conformational dynamics of RNA. In this research we mainly focus on studying tau pre-mRNA splice site hairpin and some of its mutants using optical tweezers. Single tau pre-mRNA molecules are combined with DNA/RNA hybrid handles, which can be stretched with optical tweezers, measuring the tiny force around 20 picoNewtons (pN). In this part, we constructed the tau pre-mRNA splice site hairpin flanked by DNA/RNA hybrid handles for single-molecule pulling experiment. Applying Extensible Worm-Like Chain (EWLC) model, we compared the difference of mechanical properties between wild type tau pre-mRNA and some of its mutants, revealing the difference in conformational dynamics between wild type tau pre-mRNA and its mutants.
URI: http://hdl.handle.net/10356/71744
DOI: 10.32657/10356/71744
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:SPMS Theses

Files in This Item:
File Description SizeFormat 
Thesis_SHI Jiahao_revised.pdf3.29 MBAdobe PDFThumbnail
View/Open

Page view(s)

152
Updated on May 13, 2021

Download(s) 50

31
Updated on May 13, 2021

Google ScholarTM

Check

Altmetric


Plumx

Items in DR-NTU are protected by copyright, with all rights reserved, unless otherwise indicated.