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Title: Tissue imaging using nonlinear laser scanning microscope
Authors: Seeto, David Weng Hwee
Keywords: DRNTU::Science::Physics::Optics and light
Issue Date: 2017
Abstract: Linear optics have been commonly used in microscopy around since invention. However, traditional optical imaging approaches based on linear optics have certain limitations such as out-of-focus fluorescence and limited penetration depth for bio-imaging. All these limitations can be avoided through various novel non-linear optical imaging methods, which are enabled by recent advance on ultrafast laser technology. This report aims to give an overview of how tissues can be imaged using non-linear optics approaches such as two photon excitation fluorescence and second harmonic generation. Within this report includes the principles of light, how light is perceived, the basic setup of a microscope, how fluorescence can be generated, basic setup of a fluorescence microscope, the improvisation made to fluorescent microscopes, the principle of laser operations, non-linear optics, formation of digital image, setup of a laser scanning microscope and how to align a laser system for ideal resolution.
Schools: School of Chemical and Biomedical Engineering 
Organisations: A*STAR SIMTech
Rights: Nanyang Technological University
Fulltext Permission: restricted
Fulltext Availability: With Fulltext
Appears in Collections:SCBE Student Reports (FYP/IA/PA/PI)

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