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|Title:||The role of Importin-7 (IPO7) during activity-dependent nucleocytoplasmic shuttling of transcription modulators in hippocampal neurons||Authors:||Chin, Pei Wern||Keywords:||DRNTU::Science::Biological sciences::Molecular biology||Issue Date:||2017||Abstract:||Importin-7, a member of the importin-beta superfamily of nuclear adaptor proteins, is thought to shuttle activated MAPK into the nucleus to induce transcription-dependent encoding of long-term memories in Drosophila. However, little is known about the function of importin-7, its cellular distribution, mechanisms of action and the additional cargo proteins it binds to. This project aims to characterize importin-7 in rodent hippocampal neurons with the ultimate goal of deciphering its function during learning and memory. Our initial findings indicate that importin-7 is localized at the cytosol, dendrites and spines in mature hippocampal neurons. We found that cytosolic importin- 7 undergoes activity-dependent translocation into the nucleus upon chemical long-term potentiation in acute whole brain slices. To identify cargo proteins that bind to importin-7 in the brain, we performed co-immunoprecipitation studies for importin-7 and analyzed affinity-purified proteins via mass spectrometry, but results are currently pending. Overall, our initial characterization studies hint that importin-7 could be involved in the activity-dependent transport of synaptic proteins into the nucleus during stimuli associated with transcription-dependent long-term plasticity.||URI:||http://hdl.handle.net/10356/72466||Rights:||Nanyang Technological University||Fulltext Permission:||restricted||Fulltext Availability:||With Fulltext|
|Appears in Collections:||SBS Student Reports (FYP/IA/PA/PI)|
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