Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/72647
Title: Characterization of artemisinin resistance in plasmodium falciparum
Authors: Rocamora, Frances Maureen C.
Keywords: DRNTU::Science::Biological sciences::Molecular biology
Issue Date: 2017
Source: Rocamora, F. M. C. (2017). Characterization of artemisinin resistance in plasmodium falciparum. Doctoral thesis, Nanyang Technological University, Singapore.
Abstract: Artemisinins are an important class of antimalarials because of their remarkable antimalarial activity and exceptional safety profile. However, the decline in efficacy of artemisinin-based drugs jeopardizes global efforts that aim to control and ultimately eradicate malaria. In order to better understand the Artemisinin resistance phenotype, we developed Artemisinin-resistant parasite lines derived from two isogenic clones (6A and 11C) of the 3D7 strain of Plasmodium falciparum using a selection regimen that mimics how parasites interact with the drug in infected patients. Long term in vitro selection produced two parasite lines that displayed profound stage-specific resistance to artemisinin and its relative compounds. Chemosensitivity and transcriptional profiling of ART-resistant parasites indicate that enhanced adaptive responses against oxidative stress and protein damage are associated with the artemisinin resistance phenotype. Genomic characterization of both parasite lines also identified a spectrum of mutated and copy number-variable genes that could play a role in mediating artemisinin sensitivity. Collectively, this work represents a useful resource for investigating artemisinin response and resistance in P. falciparum.
URI: http://hdl.handle.net/10356/72647
Fulltext Permission: open
Fulltext Availability: With Fulltext
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