A paper-based glucose sensoring microneedle patch

Abstract

Glucose is a significant metabolic indicator in the human body. By diagnose this index can detect many diseases like diabetes. However, the current most methods of clinical analysis of the glucose level need to pierce skin to take blood sample or takes a long time to analyze which will cause skin infection and uncomfortable of patients. MN is the well-known for the advantages of invasive, painless, safe and easy to use. In this study, we have successfully developed a paper-based colorimetric glucose sensing microneedle patch for timely glucose level analysis by rapidly extract skin interstitial fluid. This MN patch is made by methacrylated hyaluronic acid (MeHA) which will accelerate ISF extraction from the body. We have demonstrated from in vitro experiments that MN will change color gradually with time after penetration into different concentration of agarose gel, and change of color saturation with various glucose concentration after penetration for 20 minutes. In the vivo experiment, it indicated that the color value measured by paper-based glucose sensing MN Patch are consistent with the changes in blood glucose level in mice. This paper-based colorimetric glucose MN patch platform shows great potential for future minimally invasive transdermal glucose detector applications.