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https://hdl.handle.net/10356/77520
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DC Field | Value | Language |
---|---|---|
dc.contributor.author | Ling, Blysse | |
dc.date.accessioned | 2019-05-30T06:05:47Z | |
dc.date.available | 2019-05-30T06:05:47Z | |
dc.date.issued | 2019 | |
dc.identifier.uri | http://hdl.handle.net/10356/77520 | |
dc.description.abstract | Human skin has three layers: the epidermis, dermis and hypodermis. The epidermis in turn consists of four layers of increasingly-differentiated keratinocytes. Polyamines, small positively-charged molecules which can bind to the relevant DNA and RNA sequences as small positively-charged molecules and affect their expression, are essential for keratinocyte differentiation. Enzymes like AMD1 and SAT1 are involved in their creation and breakdown. Breakdown of polyamines by SAT1 releases H2O2, which has been shown to induce differentiation in keratinocytes. This lab has previously observed AMD1 upregulation in differentiating keratinocytes and a corresponding increase in SAT1 expression. This project aimed to confirm the observed AMD1 upregulation and investigate the necessity of SAT1 and polyamine catabolism in differentiation of cultured NTERT-1 human keratinocytes. While AMD1 upregulation in differentiation was confirmed through qRT-PCR and Western blotting, technical difficulties prevented any firm conclusion regarding SAT1 and polyamine catabolism. Further studies are needed in this area. | en_US |
dc.format.extent | 29 p. | en_US |
dc.language.iso | en | en_US |
dc.rights | Nanyang Technological University | |
dc.subject | DRNTU::Science::Biological sciences | en_US |
dc.title | Investigating effects of polyamine catabolism on epidermal differentiation | en_US |
dc.type | Final Year Project (FYP) | en_US |
dc.contributor.supervisor | Leah Vardy | en_US |
dc.contributor.school | School of Biological Sciences | en_US |
dc.description.degree | Bachelor of Science in Biological Sciences | en_US |
dc.contributor.organization | A*STAR, Institute of Medical Biology | en_US |
dc.contributor.organization | Skin Research Institute of Singapore | |
item.fulltext | With Fulltext | - |
item.grantfulltext | restricted | - |
Appears in Collections: | SBS Student Reports (FYP/IA/PA/PI) |
Files in This Item:
File | Description | Size | Format | |
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fyp thesis draft 1 copy.pdf Restricted Access | 756.04 kB | Adobe PDF | View/Open |
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